Need help with my writing homework on Task For each lab session start a new page in a word document. You should date the top and state briefly the aims of the tasks that you carried out that day. Then include details of the work done and any results that you gathered that day (include the res. Write a 1000 word paper answering; It was also aimed at refreshing the student’s memory on the aseptic technique and how bacteria in suspension are enumerated.
By use of the cultures and some of the plates availed, transfers were done aseptically. Transfers done included: broth to broth transfer, broth to agar plate transfer using a loop, agar to agar plate transfer and broth to broth transfer again. The numbers of the viable bacteria count were then estimated by colony count. The E. coli culture was diluted using sterile saline as diluent. Spread plate inoculation was then used. This technique enables the quantification of the number of bacteria in a solution (Wise, 2013). Miles and Misra method was also used. Its advantage is that it is very economical with the agar media (Markey, Leonard, Archambault, Cullinane and Maguire, 2013). The laboratory environment was then sampled for bacterial contaminants. The two environments sampled included air and the individuals in the laboratory. In the case of humans in the laboratory, the student in person was sampled through swabbing.
Turbidity was observed in the broth to broth transfer in one of the broths while the other one was clear. Hence, it was inferred that the wanted bacteria grew throughout the sediment at the bottom of the bottle.
As the E.coli wer transferred into the agar plate, growth of a single colony was observed on the spread plate. The colony had cells that were small, white and round in shape. The agar plate did not show any characteristics of bacterial growth. It was also noted that the bacteria that were collected were not enough with no contamination occurring.
At 25 and 37 degrees Celsius, the air plate that was placed away from the Bunsen burner showed the growth of a small single colony unlike the plate near the burner which expressed growth of five colonies of bacteria.