any expert in reading protein techniques..please message me back…
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Figure 1: Expression of Prx proteins in different tissues of Zebrafish. All lanes contain 5 μg protein. The organs are lane 1- Brain; 2- Heart; 3- Gills; 4- Liver; 5- Intestines; 6-Testes; 7- Large testes.
(A) SDS-PAGE conducted on a 10% (w/v) acrylamide gel with the indicated tissues of Zebrafish organs extracted into Zebrafish extraction buffer (10mM TrisHCl pH 7.4, 0.5 M EDTA , 5M NaCl& 10% NP40). Total protein was estimated using the method of Bradford (1976) and 5μg protein was loaded into each lane of the gel.
(B) Immunoblot analysis of a parallel gel to panel A using 1:1000 dilution of anti-(SBT-Prx) antibodies.
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B)
Figure 2: Expression of Prx4 proteins in different tissues of Zebrafish. All lanes contain 5μg protein. The organs are lane 1-Brain; lane 2- Gills; 3- Liver; 4- Intestines; 5- Muscles 6-Testes;7- Large testes.
(A) SDS-PAGE conducted on a 10% (w/v) acrylamide gel with the indicated tissues of Zebrafish organs extracted into Zebrafish extraction buffer (10mM TrisHCl pH 7.4, 0.5 M EDTA, 5M NaCl& 10% NP40). Total protein was estimated using the method of Bradford (1976) and 5μg protein was loaded into each lane of the gel.
(B) Immunoblot analysis of a parallel gel to panel A using 1:1000 dilution of anti-(human-Prx 4) antibodies.
A)
B)
Figure 2: Expression of Prx proteins in different tissues of Zebrafish. All lanes contain 5μg protein. The organs are lane 1-Brain; lane 2- Gills; 3- Liver; 4- Intestines; 5- Muscles 6-Testes;7- Large testes.
(A) SDS-PAGE conducted on a 10% (w/v) acrylamide gel with the indicated tissues of Zebrafish organs extracted into Zebrafish extraction buffer (10mM TrisHCl pH 7.4, 0.5 M EDTA, 5M NaCl& 10% NP40). Total protein was estimated using the method of Bradford (1976) and 5μg protein was loaded into each lane of the gel.
(B) Immunoblot analysis of a parallel gel to panel A using 1:1000 dilution of anti-(SBT Prx 2) antibodies and anti-(human-Prx 4) antibodies concurrently.
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B)
Figure: Expression of Prx proteins in different tissues of normal and fertile male of Zebrafish. All lanes contain 3μg protein and the first lane of each organ is represent tissue from normal males wither the second lane represent the tissue from the fertile males. The organs are lane 1-Brain; lane 2- Gills; 3- Liver; 4- Intestines; 5- Muscles 6-Testes.
(A) SDS-PAGE conducted on a 10% (w/v) acrylamide gel with the indicated tissues of Zebrafish organs extracted into Zebrafish extraction buffer (10mM TrisHCl pH 7.4, 0.5 M EDTA, 5M NaCl& 10% NP40). Total protein was estimated using the method of Bradford (1976) and 5μg protein was loaded into each lane of the gel.
(B) Immunoblot analysis of a parallel gel to panel A using 1:1000 dilution of anti-(SBT-Prx) antibodies.
A)
B)
Figure: Expression of Prx4 proteins in different tissues of Zebrafish. All lanes contain 5μg protein. The organs are lane 1-Brain; lane 2- Gills; 3- Liver; 4- Intestines; 5- Muscles 6-Testes.
(A) SDS-PAGE conducted on a 10% (w/v) acrylamide gel with the indicated tissues of Zebrafish organs extracted into Zebrafish extraction buffer (10mM TrisHCl pH 7.4, 2 mM EDTA, 150 mMNaCl, 0.125% {v/v} NP40 & 5 mM DTT). Total protein was estimated using the method of Bradford (1976) and 5μg protein was loaded into each lane of the gel.
(B) Immunoblot analysis of a parallel gel to panel A using 1:1000 dilution of anti-(human-Prx 4) antibodies.
A)
A)
B)
Figure: Expression of Prx4 proteins in human cells sample (positive control) and different fish tissues: lane 1-human cells; lane 2- SBT cells; 3- zebrafish liver; 4- zebrafish testes.
(A) SDS-PAGE conducted on a 10% (w/v) acrylamide gel with the indicated tissues of zebrafish organs extracted into zebrafish extraction buffer (10mM TrisHCl pH 7.4, 2 mM EDTA, 150 mMNaCl, 0.125% {v/v} NP40 & 5 mM DTT). Total protein was estimated using the method of Bradford (1976) and 5μg protein was loaded into each lane of the gel.
(B) Immunoblot analysis of a parallel gel to panel A using 1:1000 dilution of anti-(human-Prx 4) antibodies.
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